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Mendeley readers
Chapter title |
Mutagenesis in Newts: Protocol for Iberian Ribbed Newts
|
---|---|
Chapter number | 10 |
Book title |
TALENs
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-2932-0_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2931-3, 978-1-4939-2932-0
|
Authors |
Toshinori Hayashi, Takashi Takeuchi, Hayashi, Toshinori, Takeuchi, Takashi |
Abstract |
Newts have the remarkable capability of organ/tissue regeneration, and have been used as a unique experimental model for regenerative biology. The Iberian ribbed newt (Pleurodeles waltl) is suitable as a model animal. We have established methods for artificial insemination and efficient transgenesis using P. waltl newts. In addition to the transgenic technique, development of TALENs enables targeting mutagenesis in the newts. We have reported that TALENs efficiently disrupted targeted genes in newt embryos. In this chapter, we introduce a protocol for TALEN-mediated gene targeting in Iberian ribbed newts. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 42% |
Other | 2 | 17% |
Professor | 1 | 8% |
Researcher | 1 | 8% |
Professor > Associate Professor | 1 | 8% |
Other | 1 | 8% |
Unknown | 1 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 42% |
Biochemistry, Genetics and Molecular Biology | 3 | 25% |
Veterinary Science and Veterinary Medicine | 2 | 17% |
Unknown | 2 | 17% |