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Mendeley readers
Chapter title |
Targeted Mutagenesis in Zebrafish by TALENs
|
---|---|
Chapter number | 15 |
Book title |
TALENs
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-2932-0_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2931-3, 978-1-4939-2932-0
|
Authors |
Peng Huang, An Xiao, Xiangjun Tong, Shuo Lin, Bo Zhang, Huang, Peng, Xiao, An, Tong, Xiangjun, Lin, Shuo, Zhang, Bo |
Abstract |
Zebrafish is a valuable model organism to study vertebrate development, organ regeneration and to generate human disease models. As an important member of the arsenal of genome editing, TALE nucleases (TALENs) have implicated in broad applications in zebrafish reverse genetic studies. In this chapter, we describe the detailed protocols of TALEN-mediated genome manipulations in zebrafish, including targeted gene disruption by indel mutations, deletion of large genomic regions by using two pairs of TALENs, and precise genome modification by homologous recombination (HR). |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 35% |
Student > Ph. D. Student | 4 | 20% |
Student > Bachelor | 3 | 15% |
Student > Master | 2 | 10% |
Professor | 1 | 5% |
Other | 1 | 5% |
Unknown | 2 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 40% |
Agricultural and Biological Sciences | 4 | 20% |
Neuroscience | 3 | 15% |
Environmental Science | 2 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
Other | 0 | 0% |
Unknown | 2 | 10% |