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Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools

Overview of attention for book
Cover of 'Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools'

Table of Contents

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    Book Overview
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    Chapter 1 Construction of Modular Lentiviral Vectors for Effective Gene Expression and Knockdown.
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    Chapter 2 Development of Inducible Molecular Switches Based on All-in-One Lentiviral Vectors Equipped with Drug Controlled FLP Recombinase.
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    Chapter 3 Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
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    Chapter 4 Optimized Lentiviral Transduction Protocols by Use of a Poloxamer Enhancer, Spinoculation, and scFv-Antibody Fusions to VSV-G.
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    Chapter 5 Transduction of Murine Hematopoietic Stem Cells with Tetracycline-regulated Lentiviral Vectors.
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    Chapter 6 Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
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    Chapter 7 Production and Concentration of Lentivirus for Transduction of Primary Human T Cells.
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    Chapter 8 Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer.
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    Chapter 9 Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
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    Chapter 10 Conditional RNAi Using the Lentiviral GLTR System.
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    Chapter 11 Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
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    Chapter 12 Transient Expression of Green Fluorescent Protein in Integrase-Defective Lentiviral Vector-Transduced 293T Cell Line.
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    Chapter 13 Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
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    Chapter 14 Development of Lentiviral Vectors for Targeted Integration and Protein Delivery.
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    Chapter 15 Biogenesis and Functions of Exosomes and Extracellular Vesicles.
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    Chapter 16 Generation, Quantification, and Tracing of Metabolically Labeled Fluorescent Exosomes.
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    Chapter 17 Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
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    Chapter 18 Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
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    Chapter 19 Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
Attention for Chapter 19: Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
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Chapter title
Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
Chapter number 19
Book title
Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3753-0_19
Pubmed ID
27317187
Book ISBNs
978-1-4939-3751-6, 978-1-4939-3753-0
Authors

Ohno, Shin-Ichiro, Kuroda, Masahiko, Shin-ichiro Ohno, Masahiko Kuroda

Editors

Maurizio Federico

Abstract

Many types of cells release phospholipid membrane vesicles that are thought to play key roles in cell-cell communication, antigen presentation, and the spread of infectious agents. These membrane vesicles, derived from the late endosomes, are called exosomes. Various proteins, messenger RNAs (mRNAs), and microRNAs (miRNAs) are carried by exosomes to cells in remote locations, like a message in a bottle. Because they can protect encapsulated small RNAs from ribonucleases (RNases) in body fluid, exosomes represent ideal carriers for nucleic acid drugs. In addition, because exosomes are constructed from self components, they are predicted to have low antigenicity and toxicity, extremely important properties for carriers used in drug delivery. This article describes a protocol for using exosomes as carriers for RNA drug delivery systems.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 3%
Denmark 1 3%
Unknown 35 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 30%
Student > Master 4 11%
Other 3 8%
Researcher 3 8%
Student > Bachelor 2 5%
Other 6 16%
Unknown 8 22%
Readers by discipline Count As %
Medicine and Dentistry 10 27%
Biochemistry, Genetics and Molecular Biology 10 27%
Pharmacology, Toxicology and Pharmaceutical Science 2 5%
Immunology and Microbiology 2 5%
Agricultural and Biological Sciences 1 3%
Other 4 11%
Unknown 8 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 June 2016.
All research outputs
#18,423,400
of 23,660,057 outputs
Outputs from Methods in molecular biology
#7,522
of 13,343 outputs
Outputs of similar age
#272,250
of 396,922 outputs
Outputs of similar age from Methods in molecular biology
#776
of 1,460 outputs
Altmetric has tracked 23,660,057 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,343 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 396,922 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,460 others from the same source and published within six weeks on either side of this one. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.

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