| Chapter title |
Nucleic Acid Aptamers
|
|---|---|
| Chapter number | 19 |
| Book title |
Nucleic Acid Aptamers
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3197-2_19 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3196-5, 978-1-4939-3197-2
|
| Authors |
Felletti, Michele, Klauser, Benedikt, Hartig, Jörg S, Michele Felletti, Benedikt Klauser, Jörg S. Hartig, Felletti M, Klauser B, Hartig JS, Hartig, Jörg S. |
| Abstract |
The recent description of a new class of small endonucleolytic ribozymes termed twister opened new avenues into the development of artificial riboswitches, providing new tools for the development of artificial genetic circuits in bacteria. Here we present a method to develop new ligand-dependent riboswitches, employing the newly described catalytic motif as an expression platform in conjugation with naturally occurring or in vitro-selected aptameric domains. The twister motif is an outstandingly flexible tool for the development of highly active ribozyme-based riboswitches able to control gene expression in a ligand-dependent manner in Escherichia coli. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 1 | 8% |
| Unknown | 12 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 38% |
| Student > Master | 2 | 15% |
| Student > Ph. D. Student | 2 | 15% |
| Unspecified | 1 | 8% |
| Unknown | 3 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 31% |
| Agricultural and Biological Sciences | 3 | 23% |
| Unspecified | 1 | 8% |
| Immunology and Microbiology | 1 | 8% |
| Social Sciences | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 3 | 23% |