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Chapter title |
In Vitro and In Vivo Imaging of Fluorescent Aptamers
|
---|---|
Chapter number | 11 |
Book title |
Nucleic Acid Aptamers
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3197-2_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3196-5, 978-1-4939-3197-2
|
Authors |
Ioanna Théodorou, Nam Nguyen Quang, Karine Gombert, Benoit Thézé, Benoit Lelandais, Frédéric Ducongé, Théodorou, Ioanna, Quang, Nam Nguyen, Gombert, Karine, Thézé, Benoit, Lelandais, Benoit, Ducongé, Frédéric |
Abstract |
Fluorescence imaging techniques could be used in different ways to study the interaction of aptamers with biological systems from cell culture to animal models. Here, we present the methods developed in our laboratory for fluorescently labeled aptamers, study their internalization inside living cells using time-lapse microscopy, and monitor their biodistribution in mice bearing subcutaneous xenograft tumors using planar fluorescence imaging and fluorescence diffuse optical tomography (fDOT). |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
China | 1 | 13% |
Unknown | 7 | 88% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 50% |
Professor | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Unknown | 2 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 50% |
Agricultural and Biological Sciences | 2 | 25% |
Unknown | 2 | 25% |