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Nucleic Acid Aptamers

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Attention for Chapter 11: In Vitro and In Vivo Imaging of Fluorescent Aptamers
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Chapter title
In Vitro and In Vivo Imaging of Fluorescent Aptamers
Chapter number 11
Book title
Nucleic Acid Aptamers
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3197-2_11
Pubmed ID
Book ISBNs
978-1-4939-3196-5, 978-1-4939-3197-2
Authors

Ioanna Théodorou, Nam Nguyen Quang, Karine Gombert, Benoit Thézé, Benoit Lelandais, Frédéric Ducongé, Théodorou, Ioanna, Quang, Nam Nguyen, Gombert, Karine, Thézé, Benoit, Lelandais, Benoit, Ducongé, Frédéric

Abstract

Fluorescence imaging techniques could be used in different ways to study the interaction of aptamers with biological systems from cell culture to animal models. Here, we present the methods developed in our laboratory for fluorescently labeled aptamers, study their internalization inside living cells using time-lapse microscopy, and monitor their biodistribution in mice bearing subcutaneous xenograft tumors using planar fluorescence imaging and fluorescence diffuse optical tomography (fDOT).

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
China 1 13%
Unknown 7 88%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 50%
Professor 1 13%
Student > Doctoral Student 1 13%
Unknown 2 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 50%
Agricultural and Biological Sciences 2 25%
Unknown 2 25%