Chapter title |
Developing Aptamers by Cell-Based SELEX
|
---|---|
Chapter number | 3 |
Book title |
Nucleic Acid Aptamers
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3197-2_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3196-5, 978-1-4939-3197-2, 978-1-4939-3196-5, 978-1-4939-3197-2
|
Authors |
Silvia Catuogno, Carla Lucia Esposito, Vittorio de Franciscis, Catuogno, Silvia, Esposito, Carla Lucia, Franciscis, Vittorio de |
Abstract |
The reliable targeting of cell surface disease-associated proteins is a major challenge in chemical biology and molecular medicine. In this regard, aptamers represent a very attractive and innovative class of ligand molecules. Aptamers are generated by a reiterated in vitro procedure, named SELEX (Systematic Evolution of Ligands by Exponential enrichment). In order to generate aptamers for heavily modified cell surface-bound proteins and transmembrane receptors, the SELEX procedure has been recently adapted to the use of living cells as complex targets (referred as "cell-SELEX").Here we give an overview on the most recent advances in the field of cell-SELEX technology, providing a detailed description of the differential cell-SELEX approach that has been developed in our laboratory to identify specific signatures for human malignant glioma and non-small-cell lung cancer. The procedures used for the evaluation of binding specificity and for the preliminary identification of potential target receptors will be also described. |
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