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Mendeley readers
| Chapter title |
Label-Free Determination of the Dissociation Constant of Small Molecule-Aptamer Interaction by Isothermal Titration Calorimetry
|
|---|---|
| Chapter number | 9 |
| Book title |
Nucleic Acid Aptamers
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3197-2_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3196-5, 978-1-4939-3197-2
|
| Authors |
Marc Vogel, Beatrix Suess, Vogel, Marc, Suess, Beatrix |
| Abstract |
Isothermal titration calorimetry (ITC) is a powerful label-free technique to determine the binding constant as well as thermodynamic parameters of a binding reaction and is therefore well suited for the analysis of small molecule-RNA aptamer interaction. We will introduce you to the method and present a protocol for sample preparation and the calorimetric measurement. A detailed note section will point out useful tips and pitfalls. |
Mendeley readers
The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 1 | 3% |
| Unknown | 35 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 22% |
| Student > Ph. D. Student | 8 | 22% |
| Student > Master | 4 | 11% |
| Student > Postgraduate | 3 | 8% |
| Student > Bachelor | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 10 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 31% |
| Chemistry | 7 | 19% |
| Materials Science | 2 | 6% |
| Agricultural and Biological Sciences | 1 | 3% |
| Unspecified | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 12 | 33% |