Chapter title |
Methods in Mouse Atherosclerosis
|
---|---|
Chapter number | 6 |
Book title |
Methods in Mouse Atherosclerosis
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2929-0_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2928-3, 978-1-4939-2929-0
|
Authors |
Pineda-Torra, Inés, Gage, Matthew, de Juan, Alba, Pello, Oscar M, Inés Pineda-Torra, Matthew Gage, Alba de Juan, Oscar M. Pello, Juan, Alba, Pello, Oscar M., Juan, Alba de |
Abstract |
Macrophages are the most specialized phagocytic cells, and acquire specific phenotypes and functions in response to a variety of external triggers. Culture of bone marrow-derived or peritoneal macrophages from mice represents an exceptionally powerful technique to investigate macrophage phenotypes and functions in response to specific stimuli, resembling as much as possible the conditions observed in various pathophysiological settings. This chapter outlines protocols used to isolate and culture murine bone marrow-derived and peritoneal macrophages. Furthermore, we describe how these macrophages can be "polarized" to obtain specific macrophage subsets with special relevance to atherosclerosis. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 1% |
Spain | 1 | 1% |
China | 1 | 1% |
Unknown | 87 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 20 | 22% |
Researcher | 13 | 14% |
Student > Master | 9 | 10% |
Student > Postgraduate | 7 | 8% |
Student > Doctoral Student | 6 | 7% |
Other | 18 | 20% |
Unknown | 17 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 14 | 16% |
Medicine and Dentistry | 13 | 14% |
Agricultural and Biological Sciences | 13 | 14% |
Immunology and Microbiology | 12 | 13% |
Pharmacology, Toxicology and Pharmaceutical Science | 6 | 7% |
Other | 9 | 10% |
Unknown | 23 | 26% |