Chapter title |
Quantification of Cellular Proliferation in Mouse Atherosclerotic Lesions
|
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Chapter number | 14 |
Book title |
Methods in Mouse Atherosclerosis
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2929-0_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2928-3, 978-1-4939-2929-0
|
Authors |
José J. Fuster, Fuster, José J. |
Abstract |
Excessive cell proliferation within atherosclerotic plaques plays an important role in the progression of atherosclerosis. Macrophage proliferation in particular has become a major focus of attention in the cardiovascular field because it appears to mediate most of macrophage expansion in mouse atherosclerotic arteries. Therefore, quantification of cell proliferation is an essential part of the characterization of atherosclerotic plaques in experimental studies. This chapter describes two variants of a simple immunostaining protocol that allow for the quantification of cellular proliferation in mouse atherosclerotic lesions based on the detection of the proliferation-associated antigen Ki-67. |
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