Chapter title |
Flow Cytometric Analysis of Immune Cells Within Murine Aorta
|
---|---|
Chapter number | 11 |
Book title |
Methods in Mouse Atherosclerosis
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2929-0_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2928-3, 978-1-4939-2929-0
|
Authors |
Gjurich, Breanne N., Taghavie-Moghadam, Parésa L., Galkina, Elena V., Breanne N. Gjurich, Parésa L. Taghavie-Moghadam, Elena V. Galkina |
Abstract |
The immune system plays a critical role in the modulation of atherogenesis at all stages of the disease. However, there are many technical difficulties when studying the immune system within murine aortas. Common techniques such as PCR and immunohistochemistry have answered many questions about the presence of immune cells and mediators of inflammation within the aorta yet many questions remain unanswered due to the limitations of these techniques. On the other hand, cumulatively the flow cytometry approach has propelled the immunology field forward but it has been challenging to apply this technique to aortic tissues. Here, we describe the methodology to isolate and characterize the immune cells within the murine aorta and provide examples of functional assays for aortic leukocytes using flow cytometry. The method involves the harvesting and enzymatic digestion of the aorta, extracellular and intracellular protein staining, and a subsequent flow cytometric analysis. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 39 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 11 | 28% |
Student > Ph. D. Student | 8 | 21% |
Student > Bachelor | 4 | 10% |
Student > Master | 3 | 8% |
Professor > Associate Professor | 3 | 8% |
Other | 0 | 0% |
Unknown | 10 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 8 | 21% |
Immunology and Microbiology | 6 | 15% |
Biochemistry, Genetics and Molecular Biology | 6 | 15% |
Neuroscience | 2 | 5% |
Medicine and Dentistry | 2 | 5% |
Other | 3 | 8% |
Unknown | 12 | 31% |