Chapter title |
Wire Myography to Study Vascular Tone and Vascular Structure of Isolated Mouse Arteries
|
---|---|
Chapter number | 18 |
Book title |
Methods in Mouse Atherosclerosis
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2929-0_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2928-3, 978-1-4939-2929-0
|
Authors |
Lara del Campo, Mercedes Ferrer, Campo, Lara del, Ferrer, Mercedes |
Abstract |
Atherosclerosis is characterized by endothelial dysfunction and alterations in vascular reactivity, which can be investigated by wire myography. The method allows ex vivo monitoring of the transversal isometric tension developed by a vessel segment in response to different pathophysiological stimuli. Here we describe in detail how to use the wire myograph to evaluate endothelial function and vasoconstrictor or vasodilator properties of the vessel, as well as to identify and characterize different factors and molecular pathways that control vascular tone. We also describe how to use the wire myograph to analyze biomechanical and passive properties of vessels such as diameter and elasticity. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 57 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 10 | 18% |
Student > Bachelor | 10 | 18% |
Student > Master | 8 | 14% |
Researcher | 3 | 5% |
Professor | 3 | 5% |
Other | 7 | 12% |
Unknown | 16 | 28% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 13 | 23% |
Medicine and Dentistry | 12 | 21% |
Agricultural and Biological Sciences | 5 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 5% |
Neuroscience | 3 | 5% |
Other | 6 | 11% |
Unknown | 15 | 26% |