Chapter title |
Expansion of Human Induced Pluripotent Stem Cells in Stirred Suspension Bioreactors.
|
---|---|
Chapter number | 311 |
Book title |
Bioreactors in Stem Cell Biology
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/7651_2015_311 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6476-5, 978-1-4939-6478-9
|
Authors |
Almutawaa, Walaa, Rohani, Leili, Rancourt, Derrick E, Walaa Almutawaa, Leili Rohani, Derrick E. Rancourt |
Abstract |
Human induced pluripotent stem cells (hiPSCs) hold great promise as a cell source for therapeutic applications and regenerative medicine. Traditionally, hiPSCs are expanded in two-dimensional static culture as colonies in the presence or absence of feeder cells. However, this expansion procedure is associated with lack of reproducibility and low cell yields. To fulfill the large cell number demand for clinical use, robust large-scale production of these cells under defined conditions is needed. Herein, we describe a scalable, low-cost protocol for expanding hiPSCs as aggregates in a lab-scale bioreactor. |
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