Chapter title |
A Microfluidic Bioreactor for Toxicity Testing of Stem Cell Derived 3D Cardiac Bodies
|
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Chapter number | 340 |
Book title |
Bioreactors in Stem Cell Biology
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Published in |
Methods in molecular biology, April 2016
|
DOI | 10.1007/7651_2016_340 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6476-5, 978-1-4939-6478-9
|
Authors |
Jonas Christoffersson, Gunnar Bergström, Kristin Schwanke, Henning Kempf, Robert Zweigerdt, Carl-Fredrik Mandenius |
Abstract |
Modeling tissues and organs using conventional 2D cell cultures is problematic as the cells rapidly lose their in vivo phenotype. In microfluidic bioreactors the cells reside in microstructures that are continuously perfused with cell culture medium to provide a dynamic environment mimicking the cells natural habitat. These micro scale bioreactors are sometimes referred to as organs-on-chips and are developed in order to improve and extend cell culture experiments. Here, we describe the two manufacturing techniques photolithography and soft lithography that are used in order to easily produce microfluidic bioreactors. The use of these bioreactors is exemplified by a toxicity assessment on 3D clustered human pluripotent stem cells (hPSC)-derived cardiomyocytes by beating frequency imaging. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 8 | 31% |
Other | 4 | 15% |
Student > Bachelor | 4 | 15% |
Student > Ph. D. Student | 2 | 8% |
Student > Master | 2 | 8% |
Other | 1 | 4% |
Unknown | 5 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 23% |
Agricultural and Biological Sciences | 5 | 19% |
Chemical Engineering | 3 | 12% |
Immunology and Microbiology | 2 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Other | 3 | 12% |
Unknown | 6 | 23% |