Chapter title |
Development of a Bladder Bioreactor for Tissue Engineering in Urology
|
---|---|
Chapter number | 309 |
Book title |
Bioreactors in Stem Cell Biology
|
Published in |
Methods in molecular biology, December 2015
|
DOI | 10.1007/7651_2015_309 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6476-5, 978-1-4939-6478-9
|
Authors |
Niall F. Davis, Anthony Callanan, Davis, Niall F., Callanan, Anthony |
Abstract |
A urinary bladder bioreactor was constructed to replicate physiological bladder dynamics. A cyclical low-delivery pressure regulator mimicked filling pressures of the human bladder. Cell growth was evaluated by culturing human urothelial cells (UCs) on porcine extracellular matrix scaffolds (ECMs) in the bioreactor and in static growth conditions for 5 consecutive days. UC proliferation was compared with quantitative viability indicators and by fluorescent markers for intracellular esterase activity and plasma membrane integrity. Scaffold integrity was characterized with scanning electron microscopy and 4,6-diamidino-2-phenylindole staining. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 3 | 18% |
Researcher | 3 | 18% |
Other | 2 | 12% |
Student > Master | 2 | 12% |
Student > Doctoral Student | 1 | 6% |
Other | 2 | 12% |
Unknown | 4 | 24% |
Readers by discipline | Count | As % |
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Environmental Science | 1 | 6% |
Agricultural and Biological Sciences | 1 | 6% |
Psychology | 1 | 6% |
Medicine and Dentistry | 1 | 6% |
Other | 3 | 18% |
Unknown | 8 | 47% |