Chapter title |
Simple and Effective Affinity Purification Procedures for Mass Spectrometry-Based Identification of Protein-Protein Interactions in Cell Signaling Pathways.
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Chapter number | 13 |
Book title |
Proteomics in Systems Biology
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3341-9_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3339-6, 978-1-4939-3341-9
|
Authors |
Kwan, Julian H M, Emili, Andrew, Julian H. M. Kwan, Andrew Emili |
Editors |
Jörg Reinders |
Abstract |
Identification of protein-protein interactions can be a critical step in understanding the function and regulation of a particular protein and for exploring intracellular signaling cascades. Affinity purification coupled to mass spectrometry (APMS) is a powerful method for isolating and characterizing protein complexes. This approach involves the tagging and subsequent enrichment of a protein of interest along with any stably associated proteins that bind to it, followed by the identification of the interacting proteins using mass spectrometry. The protocol described here offers a quick and simple method for routine sample preparation for APMS analysis of suitably tagged human cell lines. |
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