| Chapter title |
Monitoring PPARG-Induced Changes in Glycolysis by Selected Reaction Monitoring Mass Spectrometry.
|
|---|---|
| Chapter number | 5 |
| Book title |
Proteomics in Systems Biology
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3341-9_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3339-6, 978-1-4939-3341-9
|
| Authors |
Andreas Hentschel, Robert Ahrends |
| Editors |
Jörg Reinders |
| Abstract |
As cells develop and differentiate, they change in function and morphology, which often precede earlier changes in signaling and metabolic control. Here we present a selected reaction monitoring (SRM) approach which allows for the parallel quantification of metabolic regulators and their downstream targets.In particular we explain and describe how to monitor abundance changes of glycolytic enzymes upon PPARγ activation by using a label-free or a stable isotope-labeled standard peptide (SIS peptides) approach applying triple-quadrupole mass spectrometry. We further outline how to fractionate the cell lysate into cytosolic and nuclear fractions to enhance the sensitivity of the measurements and to investigate the dynamic concentration changes in those compartments. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 1 | 17% |
| Professor | 1 | 17% |
| Student > Ph. D. Student | 1 | 17% |
| Researcher | 1 | 17% |
| Unknown | 2 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 33% |
| Unspecified | 1 | 17% |
| Neuroscience | 1 | 17% |
| Unknown | 2 | 33% |