| Chapter title |
From Phosphoproteome to Modeling of Plant Signaling Pathways.
|
|---|---|
| Chapter number | 18 |
| Book title |
Proteomics in Systems Biology
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3341-9_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3339-6, 978-1-4939-3341-9
|
| Authors |
Maksim Zakhartsev, Heidi Pertl-Obermeyer, Waltraud X. Schulze |
| Editors |
Jörg Reinders |
| Abstract |
Quantitative proteomic experiments in recent years became almost routine in many aspects of biology. Particularly the quantification of peptides and corresponding phosphorylated counterparts from a single experiment is highly important for understanding of dynamics of signaling pathways. We developed an analytical method to quantify phosphopeptides (pP) in relation to the quantity of the corresponding non-phosphorylated parent peptides (P). We used mixed-mode solid-phase extraction to purify total peptides from tryptic digest and separated them from most of the phosphorous-containing compounds (e.g., phospholipids, nucleotides) which enhances pP enrichment on TiO2 beads. Phosphoproteomic data derived with this designed method allows quantifying pP/P stoichiometry, and qualifying experimental data for mathematical modeling. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 43% |
| Student > Ph. D. Student | 2 | 29% |
| Unspecified | 1 | 14% |
| Lecturer | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 29% |
| Biochemistry, Genetics and Molecular Biology | 2 | 29% |
| Unspecified | 1 | 14% |
| Computer Science | 1 | 14% |
| Neuroscience | 1 | 14% |
| Other | 0 | 0% |