Chapter title |
Tissue MALDI Mass Spectrometry Imaging (MALDI MSI) of Peptides.
|
---|---|
Chapter number | 10 |
Book title |
Proteomics in Systems Biology
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3341-9_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3339-6, 978-1-4939-3341-9
|
Authors |
Birte Beine, Hanna C. Diehl, Helmut E. Meyer, Corinna Henkel |
Editors |
Jörg Reinders |
Abstract |
Matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) is a technique to visualize molecular features of tissues based on mass detection. This chapter focuses on MALDI MSI of peptides and provides detailed operational instructions for sample preparation of cryoconserved and formalin-fixed paraffin-embedded (FFPE) tissue. Besides sample preparation we provide protocols for the MALDI measurement, tissue staining, and data analysis. On-tissue digestion and matrix application are described for two different commercially available and commonly used spraying devices: the SunCollect (SunChrom) and the ImagePrep (Bruker Daltonik GmbH). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 37 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 9 | 24% |
Student > Ph. D. Student | 6 | 16% |
Student > Doctoral Student | 4 | 11% |
Researcher | 4 | 11% |
Student > Master | 3 | 8% |
Other | 4 | 11% |
Unknown | 7 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 11 | 30% |
Chemistry | 4 | 11% |
Neuroscience | 3 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 5% |
Medicine and Dentistry | 2 | 5% |
Other | 4 | 11% |
Unknown | 11 | 30% |