Chapter title |
A Simple Workflow for Large Scale Shotgun Glycoproteomics.
|
---|---|
Chapter number | 20 |
Book title |
Proteomics in Systems Biology
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3341-9_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3339-6, 978-1-4939-3341-9
|
Authors |
Guldbrandsen, Astrid, Barsnes, Harald, Kroksveen, Ann Cathrine, Berven, Frode S, Vaudel, Marc, Astrid Guldbrandsen, Harald Barsnes, Ann Cathrine Kroksveen, Frode S. Berven, Marc Vaudel |
Editors |
Jörg Reinders |
Abstract |
Targeting subproteomes is a good strategy to decrease the complexity of a sample, for example in body fluid biomarker studies. Glycoproteins are proteins with carbohydrates of varying size and structure attached to the polypeptide chain, and it has been shown that glycosylation plays essential roles in several vital cellular processes, making glycosylation a particularly interesting field of study. Here, we describe a method for the enrichment of glycosylated peptides from trypsin digested proteins in human cerebrospinal fluid. We also describe how to perform the data analysis on the mass spectrometry data for such samples, focusing on site-specific identification of glycosylation sites, using user friendly open source software. |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 15 | 100% |
Demographic breakdown
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Researcher | 4 | 27% |
Student > Master | 3 | 20% |
Professor > Associate Professor | 2 | 13% |
Student > Ph. D. Student | 2 | 13% |
Professor | 2 | 13% |
Other | 2 | 13% |
Readers by discipline | Count | As % |
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Neuroscience | 2 | 13% |
Social Sciences | 2 | 13% |
Unspecified | 1 | 7% |
Agricultural and Biological Sciences | 1 | 7% |
Other | 3 | 20% |