Chapter title |
Targeted Phosphoproteome Analysis Using Selected/Multiple Reaction Monitoring (SRM/MRM).
|
---|---|
Chapter number | 7 |
Book title |
Proteomics in Systems Biology
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3341-9_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3339-6, 978-1-4939-3341-9
|
Authors |
Adachi, Jun, Narumi, Ryohei, Tomonaga, Takeshi, Jun Adachi, Ryohei Narumi, Takeshi Tomonaga |
Editors |
Jörg Reinders |
Abstract |
Mass spectrometry-based phosphoproteomics has been rapidly spread based on the advancement of mass spectrometry and development of efficient enrichment techniques for phosphorylated proteins or peptides. Non-targeted approach has been employed in most of the studies for phosphoproteome analysis. However, targeted approach using selected/multiple reaction monitoring (SRM/MRM) is an indispensible technique used for the quantitation of known targets especially when we have many samples to quantitate phosphorylation events on proteins in biological or clinical samples. We herein describe the application of a large-scale phosphoproteome analysis and SRM-based quantitation for the systematic discovery and validation of biomarkers. |
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