| Chapter title |
Isolation of Human Skin Lymphatic Endothelial Cells and 3D Reconstruction of the Lymphatic Vasculature In Vitro
|
|---|---|
| Chapter number | 18 |
| Book title |
Lymphangiogenesis
|
| Published in |
Methods in molecular biology, September 2018
|
| DOI | 10.1007/978-1-4939-8712-2_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8711-5, 978-1-4939-8712-2
|
| Authors |
Anita Rogic, Francois Auger, Mihaela Skobe, Rogic, Anita, Auger, Francois, Skobe, Mihaela |
| Abstract |
Studies of lymphangiogenesis and lymphatic endothelial biology in vitro require pure cultures of lymphatic endothelial cells and 3D vascular constructs, which closely resemble native human lymphatic vasculature. We describe a method for the isolation of human dermal microvascular lymphatic endothelial cells and generation of a 3D lymphatic capillary network. The lymphatic vascular construct is generated by coculturing primary lymphatic endothelial cells and fibroblasts in their native matrix, without the use of synthetic scaffolds or exogenous factors. The tissue is stable over many weeks and accurately recapitulates features of human dermal lymphatic microvasculature. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 43% |
| Student > Postgraduate | 2 | 14% |
| Student > Doctoral Student | 1 | 7% |
| Researcher | 1 | 7% |
| Student > Master | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 3 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 3 | 21% |
| Medicine and Dentistry | 3 | 21% |
| Biochemistry, Genetics and Molecular Biology | 2 | 14% |
| Social Sciences | 1 | 7% |
| Unknown | 5 | 36% |