Chapter title |
Characterizing Epigenetic Changes in Endothelial Cells
|
---|---|
Chapter number | 23 |
Book title |
Lymphangiogenesis
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8712-2_23 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8711-5, 978-1-4939-8712-2
|
Authors |
Matthew T. Menendez, Courtney T. Griffin, Menendez, Matthew T., Griffin, Courtney T. |
Abstract |
Chromatin immunoprecipitation (ChIP) is an antibody-based method used to identify protein-DNA interactions and sites of protein modifications to chromatin in living cells. ChIP is a powerful method for identifying genomic sites at which epigenetic changes occur in cell types of interest because many antibodies have been developed that recognize specific epigenetic modifications of histone tails. This chapter provides detailed ChIP and subsequent polymerase chain reaction (ChIP-PCR) protocols for use in cultured endothelial cells. These protocols will allow investigators to make consistent and quantitative discoveries about epigenetic changes that occur in endothelial cells at specific genomic sites under varying treatment conditions. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 100% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 1 | 100% |