| Chapter title |
Characterization of Mouse Mesenteric Lymphatic Valve Structure and Function
|
|---|---|
| Chapter number | 7 |
| Book title |
Lymphangiogenesis
|
| Published in |
Methods in molecular biology, September 2018
|
| DOI | 10.1007/978-1-4939-8712-2_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8711-5, 978-1-4939-8712-2
|
| Authors |
Amélie Sabine, Michael J. Davis, Esther Bovay, Tatiana V. Petrova, Sabine, Amélie, Davis, Michael J., Bovay, Esther, Petrova, Tatiana V. |
| Abstract |
Intraluminal valves of collecting lymphatic vessels ensure unidirectional lymph transport against hydrostatic pressure gradient. Mouse mesentery harbors up to 800 valves and represents a convenient model for lymphatic valve quantification, high resolution imaging of different stages of valve development as well as for analysis of valve function. The protocol describes embryonic and postnatal mesenteric lymphatic vessel preparation for whole-mount immunofluorescent staining and visualization of valve organization, quantification of main morphological parameters such as valve size and leaflet length, and the quantitative assessment of functional properties of adult valves using back-leak and closure tests. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 15% |
| Student > Postgraduate | 3 | 15% |
| Student > Ph. D. Student | 3 | 15% |
| Researcher | 2 | 10% |
| Student > Doctoral Student | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 7 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 20% |
| Engineering | 4 | 20% |
| Medicine and Dentistry | 3 | 15% |
| Agricultural and Biological Sciences | 1 | 5% |
| Nursing and Health Professions | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 6 | 30% |