| Chapter title |
Dorsal Ear Skin Window for Intravital Imaging and Functional Analysis of Lymphangiogenesis
|
|---|---|
| Chapter number | 17 |
| Book title |
Lymphangiogenesis
|
| Published in |
Methods in molecular biology, September 2018
|
| DOI | 10.1007/978-1-4939-8712-2_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8711-5, 978-1-4939-8712-2
|
| Authors |
Witold W. Kilarski, Esra Güç, Melody A. Swartz, Kilarski, Witold W., Güç, Esra, Swartz, Melody A. |
| Abstract |
Postdevelopmental lymphangiogenesis occurs in chronic inflammation and wound healing, and here we describe a window preparation in the mouse ear in which lymphangiogenesis can be observed and manipulated. This model has many advantages, including access for intravital immunostaining and imaging to assess morphological features and regeneration kinetics, as well as functional assays such as lymphatic clearance. We describe five procedures: (1) the creation of a collagen-fibrin-filled window in the mouse ear as a model for regenerative lymphangiogenesis, (2) intravital immunostaining for live analysis of morphology and structure, (3) lymphatic clearance assay for functional quantification, (4) whole-mount imaging with tissue clearing for confocal imaging, and (5) postmortem lymphangiography. These procedures allow for identification of morphological and functional abnormalities in both preexisting and newly formed lymphatic vessels. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 27% |
| Student > Postgraduate | 2 | 13% |
| Other | 1 | 7% |
| Student > Bachelor | 1 | 7% |
| Unknown | 7 | 47% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 3 | 20% |
| Agricultural and Biological Sciences | 2 | 13% |
| Biochemistry, Genetics and Molecular Biology | 2 | 13% |
| Computer Science | 1 | 7% |
| Engineering | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 6 | 40% |