Chapter title |
Updates on Human Neural Stem Cells: From Generation, Maintenance, and Differentiation to Applications in Spinal Cord Injury Research
|
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Chapter number | 10 |
Book title |
Human Neural Stem Cells
|
Published in |
Results and problems in cell differentiation, January 2018
|
DOI | 10.1007/978-3-319-93485-3_10 |
Pubmed ID | |
Book ISBNs |
978-3-31-993484-6, 978-3-31-993485-3
|
Authors |
Yang D. Teng, Lei Wang, Xiang Zeng, Liquan Wu, Zafer Toktas, Serdar Kabatas, Ross D. Zafonte, Teng, Yang D., Wang, Lei, Zeng, Xiang, Wu, Liquan, Toktas, Zafer, Kabatas, Serdar, Zafonte, Ross D. |
Abstract |
Human neural stem cells (hNSCs) and human induced pluripotent stem cells (hiPSCs) have been the primary focuses in basic science and translational research as well as in investigative clinical applications. Therefore, the capability to perform reliable derivation, effective expansion, and long-term maintenance of uncommitted hNSCs and hiPSCs and their targeted phenotypic differentiations through applying chemically and biologically defined medium in vitro is essential for expanding and enriching the fundamental and technological capacities of stem cell biology and regenerative medicine. In this chapter, we systematically summarized a set of protocols and unique procedures that have been developed in the laboratories of Prof. Teng and his collaborators. These regimens have been, over the years, reproducibly and productively used to derive, propagate, maintain, and differentiate hNSCs, including those derived from hiPSCs. We emphasize the multimodal methodologies that were pioneered and established in our laboratories for characterizing functional multipotency of stem cells and its value in basic science as well as translational biomedical studies. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 2 | 29% |
Unspecified | 1 | 14% |
Student > Bachelor | 1 | 14% |
Other | 1 | 14% |
Student > Ph. D. Student | 1 | 14% |
Other | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 2 | 29% |
Unspecified | 1 | 14% |
Agricultural and Biological Sciences | 1 | 14% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 14% |
Engineering | 1 | 14% |
Other | 1 | 14% |