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Mendeley readers
Chapter title |
Fluorescence-Based Ratiometric Measurement of CRAC Channel Activity in STIM-Orai-Overexpressing HEK-293 Cells
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---|---|
Chapter number | 2 |
Book title |
The CRAC Channel
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Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8704-7_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8702-3, 978-1-4939-8704-7
|
Authors |
Shuce Zhang, Lian He, Yubin Zhou, Youjun Wang, Zhang, Shuce, He, Lian, Zhou, Yubin, Wang, Youjun |
Abstract |
Calcium influx through store-operated Ca2+ entry (SOCE), mediated by STIM-operated Orai channels, is crucial for many cellular functions. To dissect the molecular mechanisms underlying the process of STIM-Orai activation and identify regulators that modify this process, ratiometric imaging of SOCE responses in HEK cells overexpressing STIM and Orai is a routinely used method. Here we describe one commonly used procedure of monitoring SOCE activity with a ratiometric membrane-permeable dye fura-2-AM. Other ratiometric indicators suitable for SOCE measurements are also discussed. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 1 | 20% |
Student > Ph. D. Student | 1 | 20% |
Student > Master | 1 | 20% |
Researcher | 1 | 20% |
Student > Postgraduate | 1 | 20% |
Other | 0 | 0% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 40% |
Chemistry | 1 | 20% |
Engineering | 1 | 20% |
Unknown | 1 | 20% |