Chapter title |
Patch-Clamp Recording of the CRAC Channel Current in STIM-Orai Overexpressing Cells
|
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Chapter number | 1 |
Book title |
The CRAC Channel
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8704-7_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8702-3, 978-1-4939-8704-7
|
Authors |
Yiming Zhang, Sisi Zheng, Yubin Zhou, Donald L. Gill, Youjun Wang, Zhang, Yiming, Zheng, Sisi, Zhou, Yubin, Gill, Donald L., Wang, Youjun |
Abstract |
Induced by the depletion of ER calcium store, the calcium influx through calcium release-activated calcium (CRAC) channels is an ubiquitous way of Ca2+ influx for most cell types. This process is mediated by STIM protein, ER calcium sensor, and PM localized Orai calcium channels. Biophysical characterization of this STIM-Orai-mediated current, or ICRAC, with whole-cell patch-clamp technique is essential for revealing the molecular mechanisms underlying the process of STIM-Orai activation or modulation. Here we describe one commonly used procedure of monitoring CRAC activity with whole-cell patch-clamp technique. |
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