| Chapter title |
High-Resolution Imaging of STIM/Orai Subcellular Localization Using Array Confocal Laser Scanning Microscopy
|
|---|---|
| Chapter number | 15 |
| Book title |
The CRAC Channel
|
| Published in |
Methods in molecular biology, September 2018
|
| DOI | 10.1007/978-1-4939-8704-7_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8702-3, 978-1-4939-8704-7
|
| Authors |
Andras T. Deak, Benjamin Gottschalk, Emrah Eroglu, Rene Rost, Markus Waldeck-Weiermair, Wolfgang F. Graier, Roland Malli, Deak, Andras T., Gottschalk, Benjamin, Eroglu, Emrah, Rost, Rene, Waldeck-Weiermair, Markus, Graier, Wolfgang F., Malli, Roland |
| Abstract |
The expression of chimeras that consist of a fluorescent protein (FP) conjugated with a protein of interest provides the ability to visualize, track, and quantify the subcellular localization and dynamics of specific proteins in biological samples. Array confocal laser scanning microscopy is an eminently suitable technique for live-cell imaging of FP-tagged fusion proteins. Here, we describe real-time monitoring of the subcellular dynamics of the stromal-interacting molecule 1 (STIM1) and Orai1, the key protagonists of store-operated Ca2+ entry (SOCE) under resting conditions, and upon Ca2+ mobilization from the endoplasmic reticulum (ER). |
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