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Mendeley readers
| Chapter title |
Mn2+ Quenching Assay for Store-Operated Calcium Entry
|
|---|---|
| Chapter number | 4 |
| Book title |
The CRAC Channel
|
| Published in |
Methods in molecular biology, September 2018
|
| DOI | 10.1007/978-1-4939-8704-7_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8702-3, 978-1-4939-8704-7
|
| Authors |
Zui Pan, Sangyong Choi, Yanhong Luo, Pan, Zui, Choi, Sangyong, Luo, Yanhong |
| Abstract |
Store-operated Ca2+ entry (SOCE) pathway plays important roles in many cellular processes, which is largely studied by using fluorescent Ca2+ indicator, Fura-2. Extracellular Mn2+ is able to cross the plasma membrane through SOCE and quenches the fluorescence signals from Fura-2. Thus, the fluorescence quenching rate by Mn2+ composes a convenient assay to monitor the extent of SOCE. This chapter describes an experimental method of Mn2+ quenching assay for both cultured esophageal epithelial and skeletal muscle cells. It also explains how to perform a quantitative analysis of graded SOCE. |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 21% |
| Student > Doctoral Student | 3 | 16% |
| Student > Postgraduate | 3 | 16% |
| Professor | 1 | 5% |
| Other | 1 | 5% |
| Other | 2 | 11% |
| Unknown | 5 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 21% |
| Neuroscience | 3 | 16% |
| Agricultural and Biological Sciences | 3 | 16% |
| Unspecified | 1 | 5% |
| Medicine and Dentistry | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 6 | 32% |