Chapter title |
A Streamlined Approach for the Construction of Large Yeast Surface Display Fab Antibody Libraries
|
---|---|
Chapter number | 8 |
Book title |
Antibody Engineering
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8648-4_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8647-7, 978-1-4939-8648-4
|
Authors |
Simon Krah, Julius Grzeschik, Simon Rosowski, Ramona Gaa, Iris Willenbuecher, Deniz Demir, Lars Toleikis, Harald Kolmar, Stefan Becker, Stefan Zielonka, Krah, Simon, Grzeschik, Julius, Rosowski, Simon, Gaa, Ramona, Willenbuecher, Iris, Demir, Deniz, Toleikis, Lars, Kolmar, Harald, Becker, Stefan, Zielonka, Stefan |
Abstract |
Yeast surface display is a versatile platform technology for antibody discovery. Nevertheless, the construction of antibody Fab libraries typically is a tedious multistep process that involves the generation of heavy chain as well as light chain display plasmids in different haploid yeast strains followed by yeast mating. Here, we present a focused one-step Golden Gate cloning approach for the generation of yeast surface display Fab libraries that allows for simultaneous introduction of heavy-chain and light-chain variable regions into one single display vector. Thereby, the overall time as well as the materials needed for library generation can be reduced significantly. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 29 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 8 | 28% |
Student > Ph. D. Student | 7 | 24% |
Student > Master | 5 | 17% |
Student > Bachelor | 4 | 14% |
Lecturer | 1 | 3% |
Other | 2 | 7% |
Unknown | 2 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 14 | 48% |
Agricultural and Biological Sciences | 3 | 10% |
Chemical Engineering | 2 | 7% |
Immunology and Microbiology | 1 | 3% |
Medicine and Dentistry | 1 | 3% |
Other | 2 | 7% |
Unknown | 6 | 21% |