Chapter title |
Construction of Synthetic Antibody Libraries
|
---|---|
Chapter number | 5 |
Book title |
Antibody Engineering
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8648-4_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8647-7, 978-1-4939-8648-4
|
Authors |
Déborah Caucheteur, Gautier Robin, Vincent Parez, Pierre Martineau, Caucheteur, Déborah, Robin, Gautier, Parez, Vincent, Martineau, Pierre |
Abstract |
Libraries of antibody fragments displayed on filamentous phages are now a widely used approach to isolate antibodies against virtually any target. We describe a simple protocol to make large and diverse libraries based on a single or a limited number of frameworks. The approach is flexible enough to be used with any antibody format, either single-chain (scFv, VHH) or multi-chain (Fv, Fab, (Fab')2), and to target in a single step the six complementarity-determining regions-or any other part-of the antibody molecule. Using this protocol, libraries larger than 1010 can be constructed in a single week. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 33 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 15% |
Researcher | 5 | 15% |
Student > Master | 5 | 15% |
Student > Bachelor | 2 | 6% |
Professor | 2 | 6% |
Other | 4 | 12% |
Unknown | 10 | 30% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 24% |
Agricultural and Biological Sciences | 5 | 15% |
Immunology and Microbiology | 3 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 9% |
Veterinary Science and Veterinary Medicine | 2 | 6% |
Other | 2 | 6% |
Unknown | 10 | 30% |