Chapter title |
Site-Specific Radioactive Labeling of Nanobodies
|
---|---|
Chapter number | 26 |
Book title |
Antibody Engineering
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8648-4_26 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8647-7, 978-1-4939-8648-4
|
Authors |
Maxine Crauwels, Sam Massa, Charlotte Martin, Cecilia Betti, Steven Ballet, Nick Devoogdt, Catarina Xavier, Serge Muyldermans, Crauwels, Maxine, Massa, Sam, Martin, Charlotte, Betti, Cecilia, Ballet, Steven, Devoogdt, Nick, Xavier, Catarina, Muyldermans, Serge |
Abstract |
Single-domain antibody fragments, also called nanobodies (Nbs), are increasingly being used as targeting molecular tools for imaging and/or targeted radionuclide therapy. To translate these tools to the clinic, it is preferred to obtain a homogeneous, well-defined, and well-characterized product. It has been shown that Sortase A, a transpeptidase found in Staphylococcus aureus, catalyzes the site-specific conjugation between a recognition oligopeptide (LPXTG, known as sortag) and an oligoglycine functionalized probe. This versatile technique manages to couple various molecular reagents, such as biotin, fluorophores, bifunctional chelators, etc., to the target protein containing the sortag. This chapter focuses on the site-specific coupling of a bifunctional chelator (e.g., CHX-A"-DTPA) to a Nb equipped with a C-terminal sortag. The chelator conjugated to the Nb can be radiolabeled with 111In or 177Lu for SPECT imaging or targeted radionuclide therapy, respectively. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 6 | 23% |
Student > Master | 4 | 15% |
Student > Bachelor | 3 | 12% |
Professor | 2 | 8% |
Student > Ph. D. Student | 2 | 8% |
Other | 0 | 0% |
Unknown | 9 | 35% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 27% |
Veterinary Science and Veterinary Medicine | 2 | 8% |
Agricultural and Biological Sciences | 2 | 8% |
Chemistry | 2 | 8% |
Medicine and Dentistry | 1 | 4% |
Other | 1 | 4% |
Unknown | 11 | 42% |