| Chapter title |
Delivery of Superoxide Dismutase Using Cys 2 -His 2 Zinc-Finger Proteins
|
|---|---|
| Chapter number | 9 |
| Book title |
Zinc Finger Proteins
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8799-3_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8798-6, 978-1-4939-8799-3
|
| Authors |
Jia Liu, Jiangmei Li, Jie Li, Lianhui Zhu, Shaojie Wang, Xuan Wei, Peixiang Ma, Liu, Jia, Li, Jiangmei, Li, Jie, Zhu, Lianhui, Wang, Shaojie, Wei, Xuan, Ma, Peixiang |
| Abstract |
Therapeutic proteins have shown great potential in treating life-threatening diseases, but the hydrophilicity and high molecular weight hamper their passing through the cell membrane. Cell-penetrating peptide (CPP)-assisted protein delivery is a simple and efficacious strategy to promote the cellular uptake of therapeutic proteins. We recently demonstrated that the engineered Cys2-His2 zinc-finger domains possess intrinsic cell permeability, which could be leveraged for intracellular protein delivery. Here we applied this method to deliver superoxide dismutase (SOD), a therapeutic protein widely used in preclinical and clinical studies. We present a protocol for the production and delivery of zinc-finger domain-fused SOD. This protocol can be extended for delivering other therapeutic proteins. |
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