Chapter title |
Selection and Characterization of DNA Aptamers Against FokI Nuclease Domain
|
---|---|
Chapter number | 12 |
Book title |
Zinc Finger Proteins
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8799-3_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8798-6, 978-1-4939-8799-3
|
Authors |
Maui Nishio, Ayana Yamagishi, Kaori Tsukakoshi, Yoshio Kato, Chikashi Nakamura, Kazunori Ikebukuro, Nishio, Maui, Yamagishi, Ayana, Tsukakoshi, Kaori, Kato, Yoshio, Nakamura, Chikashi, Ikebukuro, Kazunori |
Abstract |
Genome editing with site-specific nucleases (SSNs) may be effective for gene therapy, as SSNs can modify target genes. However, the main limitation of genome editing for clinical use is off-target effects by excess amounts of SSNs within cells. Therefore, a controlled delivery system for SSNs is necessary. Previously we have reported on a zinc finger nuclease (ZFN) delivery system, which combined DNA aptamers against FokI nuclease domain (FokI) and nanoneedles. Here, we describe how DNA aptamers against FokI were selected and characterized for genome editing applications. |
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Demographic breakdown
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