| Chapter title |
Post-Transcriptional Gene Regulation
|
|---|---|
| Chapter number | 20 |
| Book title |
Post-Transcriptional Gene Regulation
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3067-8_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3066-1, 978-1-4939-3067-8
|
| Authors |
Geisberg, Joseph V, Moqtaderi, Zarmik, Joseph V. Geisberg, Zarmik Moqtaderi |
| Editors |
Erik Dassi |
| Abstract |
In eukaryotes, RNA polymerase II-driven transcription and processing results in the formation of numerous mRNA 3' isoforms that for any given gene may differ from one another by as little as a single nucleotide. These 3' isoforms can vary in physical properties that may affect their function and stability. Here, we outline a systematic framework to measure individual mRNA 3' isoform half-lives on a genome-wide level in S. cerevisiae. Our approach utilizes the Anchor-Away system to sequester RNA polymerase II (Pol II) in the cytoplasm followed by direct single-molecule RNA sequencing to generate a highly detailed view of 3' isoform stability under most physiological conditions without many of the adverse effects associated with commonly used alternative approaches. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 33% |
| Researcher | 3 | 33% |
| Professor | 1 | 11% |
| Student > Master | 1 | 11% |
| Student > Doctoral Student | 1 | 11% |
| Other | 0 | 0% |
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|---|---|---|
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| Agricultural and Biological Sciences | 1 | 11% |
| Neuroscience | 1 | 11% |
| Medicine and Dentistry | 1 | 11% |