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Mendeley readers
| Chapter title |
Capture and Identification of miRNA Targets by Biotin Pulldown and RNA-seq.
|
|---|---|
| Chapter number | 13 |
| Book title |
Post-Transcriptional Gene Regulation
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3067-8_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3066-1, 978-1-4939-3067-8
|
| Authors |
Shen Mynn Tan, Judy Lieberman |
| Editors |
Erik Dassi |
| Abstract |
MicroRNAs (miRNAs) are small noncoding RNAs that regulate the stability and expression of target RNAs in a sequence-dependent manner. Identifying miRNA-regulated genes is key to understanding miRNA function. Here, we describe an unbiased biochemical pulldown method to identify with high-specificity miRNA targets. Regulated transcripts are enriched in streptavidin-captured mRNAs that bind to a transfected biotinylated miRNA mimic. The method is relatively simple, does not involve cross-linking and can be performed with only a million cells. Addition of an on-bead RNase digestion step also identifies miRNA recognition elements (MRE). |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 37 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 27% |
| Researcher | 7 | 19% |
| Student > Doctoral Student | 3 | 8% |
| Student > Master | 3 | 8% |
| Student > Bachelor | 2 | 5% |
| Other | 4 | 11% |
| Unknown | 8 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 13 | 35% |
| Agricultural and Biological Sciences | 8 | 22% |
| Medicine and Dentistry | 3 | 8% |
| Immunology and Microbiology | 1 | 3% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Other | 2 | 5% |
| Unknown | 9 | 24% |