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Mendeley readers
| Chapter title |
Single-Molecule Live-Cell Visualization of Pre-mRNA Splicing.
|
|---|---|
| Chapter number | 22 |
| Book title |
Post-Transcriptional Gene Regulation
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3067-8_22 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3066-1, 978-1-4939-3067-8
|
| Authors |
Robert M. Martin, José Rino, Ana C. de Jesus, Maria Carmo-Fonseca |
| Editors |
Erik Dassi |
| Abstract |
Microscopy protocols that allow live-cell imaging of molecules and subcellular components tagged with fluorescent conjugates are indispensable in modern biological research. A breakthrough was recently introduced by the development of genetically encoded fluorescent tags that combined with fluorescence-based microscopic approaches of increasingly higher spatial and temporal resolution made it possible to detect single protein and nucleic acid molecules inside living cells. Here, we describe an approach to visualize single nascent pre-mRNA molecules and to measure in real time the dynamics of intron synthesis and excision. |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 29% |
| Student > Ph. D. Student | 3 | 21% |
| Student > Master | 2 | 14% |
| Librarian | 1 | 7% |
| Lecturer | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 2 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 50% |
| Agricultural and Biological Sciences | 1 | 7% |
| Computer Science | 1 | 7% |
| Immunology and Microbiology | 1 | 7% |
| Social Sciences | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 3 | 21% |