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Mendeley readers
Chapter title |
Genome-Wide Identification of Alternative Polyadenylation Events Using 3'T-Fill.
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Chapter number | 18 |
Book title |
Post-Transcriptional Gene Regulation
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3067-8_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3066-1, 978-1-4939-3067-8
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Authors |
Stefan Wilkening, Vicent Pelechano, Lars M. Steinmetz |
Editors |
Erik Dassi |
Abstract |
Due to the increasing appreciation of the impact of alternative polyadenylation on cellular biology, our straightforward, scalable method is of interest to any researcher studying eukaryotic transcription. In addition to high quality gene expression measurements, it precisely maps poly(A) sites and thereby permits the distinction between differential 3'UTR isoforms. As sequencing through long homopolymer stretches is not possible on the Illumina platform, we developed a method that fills up the poly(A) stretch with dTTPs before the sequencing reaction starts. |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | 8% |
Unknown | 12 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 6 | 46% |
Student > Ph. D. Student | 4 | 31% |
Student > Master | 2 | 15% |
Student > Postgraduate | 1 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 54% |
Biochemistry, Genetics and Molecular Biology | 4 | 31% |
Computer Science | 1 | 8% |
Neuroscience | 1 | 8% |