| Chapter title |
In Vitro Differentiation of Naïve CD4(+) T Cells: A Tool for Understanding the Development of Atherosclerosis.
|
|---|---|
| Chapter number | 12 |
| Book title |
Methods in Mouse Atherosclerosis
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2929-0_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2928-3, 978-1-4939-2929-0
|
| Authors |
Iborra, Salvador, González-Granado, Jose M, Salvador Iborra, Jose M. González-Granado, González-Granado, Jose M. |
| Abstract |
A complete knowledge of atherosclerosis requires a better understanding of how innate and adaptive immunity operate systemically and locally within the arterial wall. T-helper 1 (Th1) lymphocyte responses have proatherogenic effects in mice, contrasting with the responses of T regulatory cells (Tregs), which can suppress growth of atherosclerotic lesions. An imbalance in the differentiation of T-helper cells may therefore impact the development, size, and stability of atherosclerosis plaques. This chapter describes a method to isolate naïve CD4(+) T cells from atherosclerosis-prone mouse peripheral blood lymphocytes and to differentiate these CD4(+) T cells in vitro to various T helper cell lineages. These techniques allow the analysis of T lymphocytes in vitro, a necessary step in the study of molecular mechanisms involved in the inflammatory responses that trigger atherosclerosis. |
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