| Chapter title |
Biotin Switch Processing and Mass Spectrometry Analysis of S-Nitrosated Thioredoxin and Its Transnitrosation Targets
|
|---|---|
| Chapter number | 20 |
| Book title |
Nitric Oxide
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7695-9_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7694-2, 978-1-4939-7695-9
|
| Authors |
Changgong Wu, Tong Liu, Yan Wang, Lin Yan, Chuanlong Cui, Annie Beuve, Hong Li, Wu, Changgong, Liu, Tong, Wang, Yan, Yan, Lin, Cui, Chuanlong, Beuve, Annie, Li, Hong |
| Abstract |
S-Nitrosation is a key posttranslational modification in regulating proteins in both normal physiology and diverse human diseases. To identify novel therapies for human diseases linked to oxidative and nitrosative stress, understanding how cells control S-nitrosation specificity could be critical. Among the enzymes known to control S-nitrosation of proteins, thioredoxin 1 (Trx1), a conserved disulfide reductase, transnitrosates and denitrosates distinct sets of target proteins. To recognize the function of Trx1 in both normal and dysfunctional cells, S-nitrosation targets of Trx1 in different cells need to be identified. However, S-nitrosation is usually too labile to be detected directly by mass spectrometry (MS). Here we present two optimized MS techniques to identify S-nitrosated Trx1 and its transnitrosation targets, using both direct and indirect MS methods. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 1 | 14% |
| Student > Ph. D. Student | 1 | 14% |
| Professor > Associate Professor | 1 | 14% |
| Student > Doctoral Student | 1 | 14% |
| Unknown | 3 | 43% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 14% |
| Social Sciences | 1 | 14% |
| Chemistry | 1 | 14% |
| Medicine and Dentistry | 1 | 14% |
| Unknown | 3 | 43% |