Chapter title |
Real-Time Imaging of Nitric Oxide Signals in Individual Cells Using geNOps
|
---|---|
Chapter number | 3 |
Book title |
Nitric Oxide
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7695-9_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7694-2, 978-1-4939-7695-9
|
Authors |
Emrah Eroglu, Helmut Bischof, Suphachai Charoensin, Markus Waldeck-Weiermaier, Wolfgang F. Graier, Roland Malli, Eroglu, Emrah, Bischof, Helmut, Charoensin, Suphachai, Waldeck-Weiermaier, Markus, Graier, Wolfgang F., Malli, Roland |
Abstract |
Nitric oxide (NO•) is a versatile signaling molecule which regulates fundamental cellular processes in all domains of life. However, due to the radical nature of NO• it has a very short half-life that makes it challenging to trace its formation, diffusion, and degradation on the level of individual cells. Very recently, we expanded the family of genetically encoded sensors by introducing a novel class of single fluorescent protein-based NO• probes-the geNOps. Once expressed in cells of interest, geNOps selectively respond to NO• by fluorescence quench, which enables real-time monitoring of cellular NO• signals. Here, we describe detailed methods suitable for imaging of NO• signals in mammalian cells. This novel approach may facilitate a broad range of studies to (re)investigate the complex NO• biochemistry in living cells. |
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