Chapter title |
Nitric Oxide Analyzer Quantification of Plant S-Nitrosothiols
|
---|---|
Chapter number | 17 |
Book title |
Nitric Oxide
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7695-9_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7694-2, 978-1-4939-7695-9
|
Authors |
Adil Hussain, Byung-Wook Yun, Gary J. Loake, Hussain, Adil, Yun, Byung-Wook, Loake, Gary J. |
Abstract |
Nitric oxide (NO) is a small diatomic molecule that regulates multiple physiological processes in animals, plants, and microorganisms. In animals, it is involved in vasodilation and neurotransmission and is present in exhaled breath. In plants, it regulates both plant immune function and numerous developmental programs. The high reactivity and short half-life of NO and cross-reactivity of its various derivatives make its quantification difficult. Different methods based on calorimetric, fluorometric, and chemiluminescent detection of NO and its derivatives are available, but all of them have significant limitations. Here we describe a method for the chemiluminescence-based quantification of NO using ozone-chemiluminescence technology in plants. This approach provides a sensitive, robust, and flexible approach for determining the levels of NO and its signaling products, protein S-nitrosothiols. |
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Unknown | 2 | 29% |
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Unknown | 2 | 29% |