Chapter title |
Mapping Plastid Transcript Population by Circular Reverse Transcription Polymerase Chain Reaction
|
---|---|
Chapter number | 18 |
Book title |
Plastids
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8654-5_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8653-8, 978-1-4939-8654-5
|
Authors |
Florence Courtois, Livia Merendino, Courtois, Florence, Merendino, Livia |
Abstract |
During evolution of photosynthetic organisms, the genetic information provided by the internalized cyanobacteria has been transferred to the nucleus. The small genome kept by the chloroplast, the so-called plastome, displays a complex organization, comprising operons under the control of multiples promoters. In addition, polycistronic transcripts undergo multiple processing events, thus generating a complex population of mRNAs from a single gene. This chapter describes a method to investigate the diversity of the mRNA population from a single gene by circular RT-PCR. The protocol provided here allows for the simultaneous mapping of both 5' and 3' ends of the same RNA molecule. |
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