Chapter title |
Purification of Chloroplasts and Chloroplast Subfractions: Envelope, Thylakoids, and Stroma—From Spinach, Pea, and Arabidopsis thaliana
|
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Chapter number | 8 |
Book title |
Plastids
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8654-5_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8653-8, 978-1-4939-8654-5
|
Authors |
Maryse A. Block, Catherine Albrieux, Block, Maryse A., Albrieux, Catherine |
Abstract |
Chloroplasts are specific organelles of plant cells dedicated to photosynthesis and delimited by a two-membrane chloroplast envelope. Their photosynthetic function is based on the development of an operational large internal membrane network, called the thylakoids, and on enzymatic processes present in the chloroplast matrix, called the stroma. Thylakoid membranes are clearly different from the chloroplast envelope and their biogenesis is dependent on biosynthetic and transport activities specific of the chloroplast envelope. Starting with the isolation of intact chloroplasts, the method presents the separation by differential centrifugation of the three main compartments of the chloroplast: the stroma, the thylakoids, and the chloroplast envelope. Three different protocols are provided, adapted for starting leaves of spinach, Arabidopsis, and pea. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 21% |
Student > Bachelor | 3 | 21% |
Researcher | 2 | 14% |
Student > Doctoral Student | 1 | 7% |
Unknown | 5 | 36% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 29% |
Biochemistry, Genetics and Molecular Biology | 3 | 21% |
Chemistry | 1 | 7% |
Engineering | 1 | 7% |
Unknown | 5 | 36% |