Chapter title |
Imaging Plastids in 2D and 3D: Confocal and Electron Microscopy
|
---|---|
Chapter number | 7 |
Book title |
Plastids
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8654-5_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8653-8, 978-1-4939-8654-5
|
Authors |
Serena Flori, Pierre-Henri Jouneau, Benoit Gallet, Leandro F. Estrozi, Christine Moriscot, Guy Schoehn, Giovanni Finazzi, Denis Falconet, Flori, Serena, Jouneau, Pierre-Henri, Gallet, Benoit, Estrozi, Leandro F., Moriscot, Christine, Schoehn, Guy, Finazzi, Giovanni, Falconet, Denis |
Abstract |
Internal chloroplast structures present complex and various characteristics, which are still largely undetermined due to insufficient imaging investigation. Information on chloroplast morphology has traditionally been collected using light microscopy (LM), confocal laser scanning microscopy (CLSM), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) techniques. However, recent technological progresses in the field of microscopy have made it possible to visualize the internal structure of chloroplast in far greater detail and in 3D. Here we recapitulate protocols to visualize chloroplasts from Arabidopsis leaves and Phaeodactylum tricornutum cells with confocal and transmission electron microscopy together with a new technique using a focused ion beam-scanning electron microscope (FIB-SEM) allowing for 3D imaging. |
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