| Chapter title |
Isolation of Plastid Fractions from the Diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum
|
|---|---|
| Chapter number | 13 |
| Book title |
Plastids
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8654-5_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8653-8, 978-1-4939-8654-5
|
| Authors |
Alexander F. Schober, Serena Flori, Giovanni Finazzi, Peter G. Kroth, Carolina Río Bártulos, Schober, Alexander F., Flori, Serena, Finazzi, Giovanni, Kroth, Peter G., Bártulos, Carolina Río |
| Abstract |
The so-called "complex" plastids from diatoms possessing four envelope membranes are a typical feature of algae that arose from secondary endosymbiosis. Studying isolated plastids from these algae may allow answering a number of fundamental questions regarding diatom photosynthesis and plastid functionality. Due to their complex architecture and their integration into the cellular endoplasmic reticulum (ER) system, their isolation though is still challenging. In this work, we report a reliable isolation technique that is applicable for the two model diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum. The resulting plastid-enriched fractions are of homogenous quality, almost free from cellular contaminants, and feature structurally intact thylakoids that are capable to perform oxygenic photosynthesis, though in most cases they seem to lack most of the stromal components as well as plastid envelopes. |
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