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Plastids

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Cover of 'Plastids'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Primary Endosymbiosis: Emergence of the Primary Chloroplast and the Chromatophore, Two Independent Events
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    Chapter 2 Complex Endosymbioses I: From Primary to Complex Plastids, Multiple Independent Events
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    Chapter 3 Complex Endosymbioses II: The Nonphotosynthetic Plastid of Apicomplexa Parasites (The Apicoplast) and Its Integrated Metabolism
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    Chapter 4 Diversity and Plasticity of Plastids in Land Plants
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    Chapter 5 The Main Functions of Plastids
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    Chapter 6 Plastid Transient and Stable Interactions with Other Cell Compartments
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    Chapter 7 Imaging Plastids in 2D and 3D: Confocal and Electron Microscopy
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    Chapter 8 Purification of Chloroplasts and Chloroplast Subfractions: Envelope, Thylakoids, and Stroma—From Spinach, Pea, and Arabidopsis thaliana
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    Chapter 9 Isolation of Inner and Outer Membranes of the Chloroplast Envelope from Spinach and Pea
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    Chapter 10 Purification of Nongreen Plastids (Proplastids and Amyloplasts) from Angiosperms, and Isolation of Their Envelope Membranes
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    Chapter 11 In Vitro Protein Import into Isolated Chloroplasts
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    Chapter 12 Analysis of the MTL Supercomplex at Contact Sites Between Mitochondria and Plastids
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    Chapter 13 Isolation of Plastid Fractions from the Diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum
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    Chapter 14 Isolating the Plasmodium falciparum Apicoplast Using Magnetic Beads
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    Chapter 15 Extraction and Quantification of Lipids from Plant or Algae
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    Chapter 16 Quantitative Assessment of the Chloroplast Lipidome
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    Chapter 17 Determination of the DNA/RNA-Associated Subproteome from Chloroplasts and Other Plastid Types
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    Chapter 18 Mapping Plastid Transcript Population by Circular Reverse Transcription Polymerase Chain Reaction
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    Chapter 19 Bioinformatic Analysis of Chloroplast Gene Expression and RNA Posttranscriptional Maturations Using RNA Sequencing
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    Chapter 20 A Guide to the Chloroplast Transcriptome Analysis Using RNA-Seq
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    Chapter 21 A Toolkit for the Characterization of the Photoprotective Capacity of Green Algae
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    Chapter 22 Rescue of Deletion Mutants to Isolate Plastid Transformants in Higher Plants
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    Chapter 23 Genetic Analysis of Chloroplast Biogenesis, and Function and Mutant Collections
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    Chapter 24 Generation of Mutants of Nuclear-Encoded Plastid Proteins Using CRISPR/Cas9 in the Diatom Phaeodactylum tricornutum
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    Chapter 25 In Silico Tools for the Prediction of Protein Import into Secondary Plastids
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    Chapter 26 AT_CHLORO: The First Step When Looking for Information About Subplastidial Localization of Proteins
Attention for Chapter 18: Mapping Plastid Transcript Population by Circular Reverse Transcription Polymerase Chain Reaction
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Chapter title
Mapping Plastid Transcript Population by Circular Reverse Transcription Polymerase Chain Reaction
Chapter number 18
Book title
Plastids
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8654-5_18
Pubmed ID
Book ISBNs
978-1-4939-8653-8, 978-1-4939-8654-5
Authors

Florence Courtois, Livia Merendino, Courtois, Florence, Merendino, Livia

Abstract

During evolution of photosynthetic organisms, the genetic information provided by the internalized cyanobacteria has been transferred to the nucleus. The small genome kept by the chloroplast, the so-called plastome, displays a complex organization, comprising operons under the control of multiples promoters. In addition, polycistronic transcripts undergo multiple processing events, thus generating a complex population of mRNAs from a single gene. This chapter describes a method to investigate the diversity of the mRNA population from a single gene by circular RT-PCR. The protocol provided here allows for the simultaneous mapping of both 5' and 3' ends of the same RNA molecule.

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Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Professor 2 40%
Researcher 1 20%
Unknown 2 40%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 40%
Unknown 3 60%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 July 2018.
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#18,641,800
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Outputs from Methods in molecular biology
#7,988
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#330,872
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Outputs of similar age from Methods in molecular biology
#950
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