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Preface. Bacterial pangenomics.

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Cover of 'Preface. Bacterial pangenomics.'

Table of Contents

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    Book Overview
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    Chapter 1 Pulsed Field Gel Electrophoresis and Genome Size Estimates
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    Chapter 2 Comparative analyses of extrachromosomal bacterial replicons, identification of chromids, and experimental evaluation of their indispensability.
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    Chapter 3 Choice of Next-Generation Sequencing Pipelines
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    Chapter 4 The pyrosequencing protocol for bacterial genomes.
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    Chapter 5 Bacterial Metabarcoding by 16S rRNA Gene Ion Torrent Amplicon Sequencing.
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    Chapter 6 The illumina-solexa sequencing protocol for bacterial genomes.
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    Chapter 7 High-throughput phenomics.
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    Chapter 8 Comparative Analysis of Gene Expression: Uncovering Expression Conservation and Divergence Between Salmonella enterica Serovar Typhimurium Strains LT2 and 14028S
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    Chapter 9 Raw sequence data and quality control.
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    Chapter 10 Methods for Assembling Reads and Producing Contigs
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    Chapter 11 Mapping Contigs Using CONTIGuator.
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    Chapter 12 Gene Calling and Bacterial Genome Annotation with BG7.
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    Chapter 13 Defining orthologs and pangenome size metrics.
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    Chapter 14 Robust Identification of Orthologues and Paralogues for Microbial Pan-Genomics Using GET_HOMOLOGUES: A Case Study of pIncA/C Plasmids.
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    Chapter 15 Genome-scale metabolic network reconstruction.
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    Chapter 16 From pangenome to panphenome and back.
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    Chapter 17 Genome-Wide Detection of Selection and Other Evolutionary Forces
  19. Altmetric Badge
    Chapter 18 The integrated microbial genome resource of analysis.
  20. Altmetric Badge
    Chapter 19 Erratum to: Genome-Wide Detection of Selection and Other Evolutionary Forces
Attention for Chapter 4: The pyrosequencing protocol for bacterial genomes.
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Chapter title
The pyrosequencing protocol for bacterial genomes.
Chapter number 4
Book title
Bacterial Pangenomics
Published in
Methods in molecular biology, October 2014
DOI 10.1007/978-1-4939-1720-4_4
Pubmed ID
Book ISBNs
978-1-4939-1719-8, 978-1-4939-1720-4
Authors

Rizzi E, Ermanno Rizzi, Rizzi, Ermanno

Abstract

The pyrosequencing methodology was applied in 2005 by 454 Lifesciences to the emerging field of next generation sequencing (NGS), revolutionizing the way of DNA sequencing. In the last years the same strategy grew up and was technologically updated, reaching a high throughput in terms of amount of generated sequences (reads) per run and in terms of length of sequence up to values of 800-1,000 bases. These features of pyrosequencing perfectly fit to bacterial genome sequencing for the de novo assemblies and resequencing as well. The approaches of shotgun and paired ends sequencing allow the bacterial genome finishing providing a high-quality data in few days with unprecedented results.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 38%
Student > Doctoral Student 2 15%
Professor > Associate Professor 2 15%
Student > Ph. D. Student 2 15%
Other 1 8%
Other 0 0%
Unknown 1 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 54%
Immunology and Microbiology 2 15%
Biochemistry, Genetics and Molecular Biology 2 15%
Veterinary Science and Veterinary Medicine 1 8%
Unknown 1 8%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 October 2014.
All research outputs
#14,203,052
of 22,768,097 outputs
Outputs from Methods in molecular biology
#4,174
of 13,090 outputs
Outputs of similar age
#134,895
of 260,259 outputs
Outputs of similar age from Methods in molecular biology
#23
of 126 outputs
Altmetric has tracked 22,768,097 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one has gotten more attention than average, scoring higher than 64% of its peers.
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We're also able to compare this research output to 126 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 76% of its contemporaries.