Chapter title |
The illumina-solexa sequencing protocol for bacterial genomes.
|
---|---|
Chapter number | 6 |
Book title |
Bacterial Pangenomics
|
Published in |
Methods in molecular biology, October 2014
|
DOI | 10.1007/978-1-4939-1720-4_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1719-8, 978-1-4939-1720-4
|
Authors |
Hu Z, Cheng L, Wang H, Zhenfei Hu, Lei Cheng, Hai Wang, Hu, Zhenfei, Cheng, Lei, Wang, Hai |
Abstract |
Based on reversible dye-terminators technology, the Illumina-solexa sequencing platform enables rapid sequencing-by-synthesis (SBS) of large DNA stretches spanning entire genomes, with the latest instruments capable of producing hundreds of gigabases of data in a single sequencing run. Illumina's NGS instruments powerfully combine the flexibility of single reads with short- and long-insert paired-end reads, and enable a wide range of DNA sequencing applications. Here, we describe the paired-end library preparation with an average insert size of 470 bp, 2 kbp, and 6 kbp, together with the DNA cluster generation and sequencing procedure of E. coli O104:H4 genome on Illumina Hiseq 2000 platform. |
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