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G Protein-Coupled Receptors in Drug Discovery

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Cover of 'G Protein-Coupled Receptors in Drug Discovery'

Table of Contents

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    Book Overview
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    Chapter 1 Purification of Stabilized GPCRs for Structural and Biophysical Analyses
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    Chapter 2 Purification and Crystallization of a Thermostabilized Agonist-Bound Conformation of the Human Adenosine A 2A Receptor
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    Chapter 3 2D Projection Analysis of GPCR Complexes by Negative Stain Electron Microscopy
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    Chapter 4 Nuts and Bolts of CF 3 and CH 3 NMR Toward the Understanding of Conformational Exchange of GPCRs
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    Chapter 5 Single-Molecule Fluorescence Microscopy for the Analysis of Fast Receptor Dynamics
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    Chapter 6 Quantitative Multi-color Detection Strategies for Bioorthogonally Labeled GPCRs
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    Chapter 7 Approaches to Characterize and Quantify Oligomerization of GPCRs
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    Chapter 8 Monitoring G Protein Activation in Cells with BRET
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    Chapter 9 Use of Fluorescence Indicators in Receptor Ligands
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    Chapter 10 Detection and Quantification of Intracellular Signaling Using FRET-Based Biosensors and High Content Imaging
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    Chapter 11 The Measurement of Receptor Signaling Bias
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    Chapter 12 Approaches to Assess Functional Selectivity in GPCRs: Evaluating G Protein Signaling in an Endogenous Environment
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    Chapter 13 Bioluminescence Resonance Energy Transfer Approaches to Discover Bias in GPCR Signaling
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    Chapter 14 Virus-Mediated Expression of DREADDs for In Vivo Metabolic Studies
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    Chapter 15 High-Throughput Screening for Allosteric Modulators of GPCRs
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    Chapter 16 Radioligand Binding Assay for an Exon 11-Associated Mu Opioid Receptor Target
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    Chapter 17 Docking and Virtual Screening Strategies for GPCR Drug Discovery
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    Chapter 18 The Dynamic Process of Drug–GPCR Binding at Either Orthosteric or Allosteric Sites Evaluated by Metadynamics
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    Chapter 19 Experiment-Guided Molecular Modeling of Protein–Protein Complexes Involving GPCRs
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    Chapter 20 Interaction Fingerprints and Their Applications to Identify Hot Spots
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